Fig. 2
MoMo30-plant is heat stable and stays bound to the virus for long periods. A MoMo30-plant was mixed with HIV-1NL43 (equivalent to 1 ng p24) at concentrations of 0.1 (red) and 1 (blue) nM sufficient to cause 50% (blue) or 70% inhibition (red). The mixture was then heated at temperatures from 25° to 120° for 30 min, allowed to cool to 25 °C. An aliquot of 100 µL was then tested in the MAGI infectivity assay, B MoMo30-plant (3 nM) was mixed with HIV-1NL43 (equivalent to 1 ng p24) and allowed to interact for 5 min before centrifuging the complex through a 40% sucrose cushion to remove from free MoMo30-plant. The virus-MoMo30-plant complex (in the pellet) was removed from 5 min to 72 h at 4 °C before testing by MAGI cell assay. All measurements were done in triplicate