Fig. 6

ASFV replication was regulated by DEPs between PSS and NPSS. MA104 cells were plated in 12 well plates at 80% confluency and transfected with the empty vector pCDNA3.1 (4 µg) or Myc-PCNA (4 µg), Myc-MASP1 (4 µg), or Myc-BST2 (4 µg). At 24 h after transfection, ASFV (MOI = 1) was added to the cells. Samples were collected at 12, 24, 36, and 48 h post infection. One sample was used to detect the genomic copies of overexpressed Myc-PCNA (A), Myc-MASP1 (B), and Myc-BST2 (C) at different ASFV infection timepoints in absolute quantitative experiments; the other sample was used to detect the effect of Myc-PCNA, Myc-MASP1, and Myc-BST2 overexpression on ASFV P72 and P30 protein expression using western blot. The group transfected with the empty vector pCDNA3.1 was used as control. The gray value analysis was performed using the ImageJ software. The data are presented as mean ± standard deviation values of three independent experiments. * indicating p value of < 0.05 denoted statistical significance; ** indicating p value of < 0.01 denoted high statistical significance