TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling

Table 2 Overview of the results of two sequencing runs of eight HPV-positive replicated samples

Sample ID	# of trimmed reads (mil)		# of reads mapped to HPV (mil)		# of reads mapped to target (mil)		Mean coverage		% Genome covered by minimum 100x		% Genome covered by minimum 300x
Sample ID	Run1	Run2	Run1	Run2	Run1	Run2	Run1	Run2	Run1 (%)	Run2 (%)	Run1 (%)	Run2 (%)
1-HPV51	10.8	4.5	2.8	0.8	2.8	0.8	27,662	8865	100	100	97	99
2-HPV52	5.1	2.2	0.5	0.2	0.5	0.2	5444	2872	100	100	96	97
3-HPV52	6.3	3.5	4.7	2.6	4.7	2.6	50,735	29,829	100	100	100	100
4-HPV51	12.6	3.3	8.4	1.3	8.4	1.3	71,702	13,628	100	100	100	98
5-HPV52	4.9	0.9	0.6	0.1	0.6	0.1	5834	1373	100	100	95	84
6-HPV52	5.9	2.5	0.8	0.4	0.8	0.4	8500	4124	100	100	98	99
7-HPV51	10.0	2.5	5.4	0.8	5.4	0.8	52,417	8402	100	100	100	97
8-HPV52	9.5	4.5	8.8	3.0	8.8	3.0	82,704	32,611	100	100	100	100

ISSN: 1743-422X