Fig. 2

RT-PCR with primers specific for Snake River alfalfa virus. M, 1 kb plus DNA marker (Thermo Fisher Scientific, Waltham, MA). Lanes 1,2: RT-PCR products amplified with primers LN1036/37 and ANPV_3, respectively. Lanes 3,4 and 5: amplification from alfalfa samples containing no SRAV reads, water control, and Taq DNA polymerase (no RT mix added to verify the absence of genomic DNA), respectively; primers LN1036/37. Lanes 6, 7, and 8: representative RT-PCR products amplified from seeds of alfalfa cultivars SW-9215, CUF101, and Maverick using LN1036/37 primers. Lanes 9,10, and 11: the same reaction controls as shown in lanes 3–5