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Fig. 5 | Virology Journal

Fig. 5

From: Mutagenesis of the di-leucine motif in the cytoplasmic tail of newcastle disease virus fusion protein modulates the viral fusion ability and pathogenesis

Fig. 5

LL-motif mutations affected the expression of F protein at the cell surface. A The expression of transfeced F protein at the cell membrane. BSR-T7/5 cells were transfected with different LL-motif mutant plasmids, and the protein was extracted and the level determined at 24 hpi. F protein expression levels of LL-motif mutants are expressed as percentages of the levels for F, which were set at 100%. B and C Cell surface expression of the F protein of LL-motif mutants. BSR-T7/5 cells were infected with rSG10* or rSG10*-F/L537A at an MOI of 0.1. The cell surface expression of F protein was determined by western blotting (B) and flow cytometry assay (C) at 36 hpi. F protein expression levels of LL-motif mutants are expressed as percentages of the levels for rSG10*, which were set at 100%. P values were calculated with a one way or two-way ANOVA; n = 3; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001

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Virology Journal

ISSN: 1743-422X