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Fig. 1 | Virology Journal

Fig. 1

From: Mutagenesis of the di-leucine motif in the cytoplasmic tail of newcastle disease virus fusion protein modulates the viral fusion ability and pathogenesis

Fig. 1

Effect of LL-motif mutations on the F protein synthesis and syncytium formation after transfection. The F protein synthesis of LL-motif mutants was detected by IFA (A) or western blotting (B). BSR-T7/5 cells were transfected with different LL-motif mutant plasmids and the cell lysates were collected at 36 or 48 hpi. F protein expression levels of LL-motif mutants are expressed as percentages of the levels for F, which were set at 100%. C The size of the syncytium induced by LL-motif mutants. After transfection with different LL-motif mutant plasmids, the BSR-T7/5 cells were stained with Giemsa solution to determine the size of the syncytium. The average syncytium diameters of wild F transfected group was set at 100%, and the syncytium diameter of LL-motif mutations were expressed as fusion index, which means the relative percentages to the wild F group. P values were calculated with a one-way ANOVA; n = 3; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001

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Virology Journal

ISSN: 1743-422X