Skip to main content

Table 2 Absolute gene copy number per reaction determined by digital PCR

From: Development and validation of a duplex real-time PCR for the rapid detection and quantitation of HTLV-1

Sample

Gene copy no./rnx

HTLV-1 gene

RPPH1 gene

Ratio of HTLV/RPPH1

Copy no./cell

pcDNA-pol-tax-RPPH1

79,800

77,800

1.001

/

100% Hut102

14,340

25,020

0.5735

1.146

4% Hut102/Jurkat

569

37,640

0.0151

0.03025

0.032% Hut102/Jurkat

5.2

39,240

0.00013

0.00026

Jurkat

0

46,720

0

0

Back to article page

Virology Journal

ISSN: 1743-422X