Fig. 5
From: Reactive oxygen species enhance rAAV transduction by promoting its escape from late endosomes
Effects of ROS on the intracellular distribution of rAAV2. A Binding assay was performed with vector-transfected Hela cells with/without heparin administration. B Internalization assay was conducted with vector-transfected Hela cells with/without heparin administration. C qPCR analysis of ss-rAAV2 vector genomes content in the cytoplasm after Hela cell pretreated with H2O2. D qPCR analysis of ss-rAAV2 vector genomes content in the nucleus after Hela cell pretreated with H2O2. E Subcellular localization of ss-rAAV2-TAMRA vectors after pre-treatment with H2O2. Hela Cells were fixed at 1 h, 5 h, or 12 h post-transduction. The localization of ss-rAAV2-TAMRA vectors was determined using confocal microscopy. TAMRA and DAPI signals are represented in red and blue, respectively. Data represent means ± SD (n = 3). ns p > 0.05; *p < 0.05 vs. PBS; ****p < 0.0001