Fig. 10
From: Reactive oxygen species enhance rAAV transduction by promoting its escape from late endosomes
Effects of endosome acidification on cathepsins B, cathepsins L, or ROS-enhanced rAAV2 transduction. pcDNA3.1-CTSB, pcDNA3.1-CTSL was transfected into HeLa cells for 24 h or pretreated with bafilomycin A1, and then H2O2 transduced with rAAV2-GFP at 1000 vg/cell. A Effects of acidic endosomal environment on ROS-promoted rAAV transgene expression. B GFP level was determined 48 h after infection. C Quantification of rAAV2 in late endosomes when rAAV2-GFP was transfected into HeLa cells for a 7-h period. Data are indicated by means ± SD (n = 3). ns p > 0.05; *p < 0.05; **p < 0.01; ****p < 0.0001