Fig. 4
Evaluation of various HC-Pros in terms of their efficiency in inhibiting RISC activity in vitro. a In vitro RISC assay (i) and evaluation of the AGO1-IP efficiency (ii) for P1/HC-ProTu and P1/HC-ProTu/atg8age plants. In: input; IP: immunoprecipitation; FT: flow through. b Comparison of the in vitro RISC efficiency among the Col-0, P1/HC-ProTu, and P1/HC-ProTu/atg8age plants. c Represented in vitro RISC assay with the P1/HC-ProTu, P1/HC-ProZy, P1/HC-ProTe, and P1/HC-ProTu−K plants from 3 biological repeats. d Relative RISC cleavage efficiency of the P1/HC-ProTu, P1/HC-ProZy, and P1/HC-ProTe plants (i) and comparison of the RISC efficiency between Col-0 and P1/HC-ProTu−K plants (ii). The bars represent the standard deviations (n = 3). Means were compared by Tukey’s honestly significance test. Different letters above the bars indicate significant differences