Development of a quadruple PCR-based gene microarray for detection of vaccine and wild-type classical swine fever virus, African swine fever virus and atypical porcine pestivirus

Table 2 Primers of CSFV, ASFV, APPV and beta-globin used in this study

Name of Primers^a	Primer sequence (5′ → 3′)	Target gene	Product size (bp)
CSFV-W-F	GGAGGGACTAGCCRTAGTG	5′UTR	77
CSFV-W-R	ACGTCGAACTACTGACGACTG-biotin	5′UTR	77
CSFV-V-F	CCTTCGGGGAGAAAGTAACGAT	NS5B	97
CSFV-V-R	CCTACCACAGTCACGGCT-biotin	NS5B	97
ASFV-F	TATATTGGCCCAAGACTTGCT	B646L	119
ASFV-R	GCACCAAATGTGTTTCTTCGAT-biotin	B646L	119
APPV-F	CAGACGTCACCGAGTAGTACACC -biotin	5′UTR	134
APPV-R	CCCAGGTCCACCACCGAT	5′UTR	134
IC-F	AAGTCTGCCGTTACTGCC-biotin	Beta-globin	83
IC-R	TAACCTTGATACCAACCTGC	Beta-globin	83

^aCSFV-W-P: probe for detection of wild type CSFV; CSFV-V-P: probe for detection of CSFV vaccine strains

ISSN: 1743-422X