Fig. 15

Establishment of a TRIM22 overexpression plasmid. A Results of agarose gel electrophoresis of the restriction enzyme-digested vector; 1 LV-007 Vector linearized by Nhe I and Age I double digestion, 2 LV-007 Vector (vector plasmid without digestion); B Lv-007 vector map, and Nhe I and Age I enzyme digestion. The component order is CMV-MCS-EF1-copGFP-T2A-puromycin; The fluorescent tags is copGFP. C Electrophoresis of the TRIM22 PCR products. A specific band at ~ 1497 bp, which was consistent with the expected size, was noted. D 1–4 positive transformants of the TRIM22 overexpression plasmid; 5 negative control (no-load self-connecting control group). The negative control was used to show no false positives in the amplification process using empty vectors without the target gene as templates. The PCR product size of the negative transformant was 312 bp; 6 Negative control (ddH2O). E False positives result from the contamination of external nucleic acid in the exclusion system