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Fig. 3 | Virology Journal

Fig. 3

From: Detection of cytomegalovirus (CMV) by digital PCR in stool samples for the non-invasive diagnosis of CMV gastroenteritis

Fig. 3

The performance estimation of CMV DNA segment detection by dPCR. A Comparison of the CMV DNA segment copy number detected and that predicted. The x-axis shows the predicted CMV DNA segment copy number of the standards, which can be estimated by the dilution degree from the raw solution with a known concentration of the CMV DNA segment. The y-axis shows the exact detected CMV DNA segment copy number of the standards. Each red triangle represents one detection, and each dilution degree corresponds to three red triangles. The green line represents the best fit curve. B The detection probability of CMV DNA segment at low concentrations. The x-axis shows the plasmid CMV DNA segment concentration, and the y-axis shows the detection probability. The red triangle represents the detection probability calculated by dividing the total number of experiments by the number of positively detected experiments. The green line represents the best nonlinear fit curve

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Virology Journal

ISSN: 1743-422X