Fig. 6
From: A high-throughput neutralizing assay for antibodies and sera evaluation against Epstein-Barr virus
Akata-EBV-GFP virus production and TEM detection. A Images of Akata-EBV cells carrying Akata-EBV-GFP before induction with goat anti-human IgG. Few GFP spots were observed indicating that most viruses were in a latent state. B Images of Akata-EBV cells carrying Akata-EBV-GFP 72 h after induction. GFP spots were readily observed in most cells indicating viruses were induced to lytic state. (A, B). Images of cells with the same field of view captured at FITC channel (left panel) and bright-field channel (right panel). C TEM images of Akata-EBV-GFP virus. Capsids are visible, but viral envelope did not resist sample processing for TEM. Capsids are visible, but the viral envelope is not observed because sample processing for TEM observation disrupted this membrane structure. The magnification of the left channel is 150,000 x (scale bar: 100 nm) and that of the right channel is 250,000 x (scale bar: 50 nm)