Fig. 5

HPV16 E7 alters POLη and TopBP1 induction in response to replication stress-inducing agents. Representative immunoblots of vector control (LXSN) and HPV16 E7 wild type and mutant E7 expressing HFKs probed for TopBP1 (A–C) or POLη (D–F). Whether these cells were exposed to the indicated replication stress-inducing agent (Hydroxyurea or HU, UV, and Cisplatin or CISP) is indicated by “−“ (not exposed) and “ + ” (exposed). GAPDH was used as a loading control