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Fig. 7 | Virology Journal

Fig. 7

From: Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Fig. 7

Disruption of the ethylene pathway in Nicotiana benthamiana makes it susceptible to infection with the cysteine-rich protein of the Chinese isolate of chrysanthemum virus B (CVB-CN CRP). a Silencing efficiency of virus-induced gene silencing (VIGS) assays was assessed by qRT-PCR at 10 days post inoculation (dpi). The data are given as means ± standard deviation of three biological replicates. The values represent relative mRNA levels against control groups (TRV:GFP-infiltrated N. benthamiana seedlings), values of which are set to 100%. Significant differences in expression are indicated with asterisks: (*) P < 0.05 or (**) P < 0.01; Tukey′s test. b Disease symptoms caused by PVX:CVB-CN CRP in the ethylene pathway impaired N. benthamiana seedlings at 9 dpi. TRV:GFP plants-inoculated with PVX:CRV-CN CRP were used as the control. c 1-Aminocyclopropane-1-carboxylic acid (ACC) content and relative viral RNA accumulation in the ethylene pathway-impaired N. benthamiana seedlings, as described in b. The data are given as means ± standard deviation of three biological replicates. The values represent ACC content or relative viral RNA levels against control groups (TRV:GFP-infiltrated N. benthamiana seedlings infected with PVX:CVB-CN CRP). Significant differences in expression are indicated with asterisks: (*) P < 0.05 or (**) P < 0.01; Tukey′s test

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Virology Journal

ISSN: 1743-422X