Fig. 2
Cytotoxicity and anti-EV-A71 activity of Aβ1–42 in vitro. a Cytotoxicity of Aβ1–42 to multiple cell lines were determined by CCK assay at 48 h post-peptide treatment. b and c SH-SY5Y cells were infected with EV-A71 (MOI = 1) and followed by treatment with Aβ1–42 peptides or RBV (20 µg/mL) for 24 h. The concentrations of EV-A71 VP1 RNA and protein were assayed by qRT-PCR and WB, respectively. d Aβ1–42 peptides or RBV were added to EV-A71-infected Vero (MOI = 0.1) and RD cells (MOI = 0.01) for 24 h. The content of EV-A71 VP1 protein was analyzed by WB. Software “Gel-Pro analyzer” was used to analysis of the optical density ratio of the bands