Fig. 1
Aβ1–42 generation and aggregation were responsive for EV-A71 infection in SH-SY5Y cells. a Production of Aβ1–42 from the supernatant was quantified with an ELISA kit. SH-SY5Y cells were subjected to EV-A71 infection (MOI = 1) and collected at 6 or 12 h post-EV-A71 infection. b Production of intracellular Aβ1–42 was quantified by IFA assay. SH-SY5Y cells were subjected to EV-A71 infection at MOIs of 0.01, 0.1, and 1 for 8 h, respectively. IFA of Aβ1–42 protein was performed with an Alexa Fluor 488-conjugated antibody (green), and the nucleus was dyed with DAPI (blue). c Aβ1–42 high-molecular-weight oligomer accumulation in response to EV-A71 (MOI = 1) at 6 and 12 h after infection was detected by Western blot with anti-Aβ1–42 antibody (~ 70 kDa)