Table 1 The oligonucleotides used for RT-RAA and crRNA in RT-RAA-Cas12a-based assay for norovirus genotype GII.4 and GII.17 detection
Assay | Name | Oligonucleotide sequences (5′-3′) |
|---|---|---|
RT-RAA | NOV-F1 | GTGCCCAGACAAGAGYCAATGTTCAGATGGATG |
NOV-F2 | CAATGTTCAGATGGATGAGRTTCTCAGATC | |
NOV-R1 | ACCATTGTACATYCTKGMCAAATGAG | |
NOV-R2 | ATGAGMMARRTARGGATTCAAATCAGGGCC | |
Cas12-based detection | crRNA1-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATAGATTGCGATCGCCCTCCCA |
crRNA1-R | TGGGAGGGCGATCGCAATCTATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
crRNA2-1-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTTGTGAATGAAGATGGCGTC | |
crRNA2-1-R | GACGCCATCTTCATTCACAAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
crRNA2-2-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTGAATGAAGATGGCGTCGA | |
crRNA2-2-R | TCGACGCCATCTTCATTCAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
crRNA3-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTTCTAATCCAGGGGTCAATT | |
crRNA3-R | AATTGACCCCTGGATTAGAAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC |