Fig. 5

The effect of various crRNA2-2 concentrations on the performance of RT-RAA-Cas12a-based assay. a The RT-RAA-Cas12a-based fluorescence readouts were measured after incubation with 0, 5, 10, 15, 20, 25 and 30 nM of crRNA2-2 for 15 min and 10 min at 39 °C, respectively. There was an obvious increase in fluorescence at the concentration of crRNA2-2 between 5 and 20 nM, while the fluorescence intensities remained almost constant with the further increase of crRNA2-2 from 20 to 30 nM. b To test whether high concentrations of crRNA2-2 could cause false positive results, different crRNA2-2 concentrations were examined using lateral flow strip (LFS) readouts only in the absence of the amplification products in RT-RAA-Cas12a-based assay. No false-positive results were found at high concentrations of crRNA2-2 such as 20 nM and 30 nM