Fig. 5
From: Detection and discovery of plant viruses in soybean by metagenomic sequencing
Symptoms caused by ClYVV-IA-2016 and ClYVV-IA-2017 in soybean. Healthy mock-inoculated controls are indicated. N. benthamiana infected with ClYVV-IA-2016 or ClYVV-IA-2017 was used as an inoculum to infect soybean plants by mechanical inoculation. A Whole plants infected with ClYVV IA-2016 at 35Â days post inoculation (dpi). B Systemic leaf symptoms at 35 dpi. A representative young leaflet is shown in the middle of the panel while mature older leaflet is shown on the right. C Whole plants infected ClYVV IA-2017 at 21 dpi. D Systemic leaf symptoms at 21 dpi. A representative young trifoliolate leaf is shown in the middle of the panel while a mature older leaflet is shown on the right. E RT-PCR detection of ClYVV isolates in soybean infected plants. Agarose gel electrophoresis of PCR confirming the presence of ClYVV-IA-2016 and ClYVV-IA-2017 identified by RNA sequencing. Lane 1: Mock- inoculated control; 2: Detection of ClYVV-IA-2016; 3: Detection of ClYVV-IA-2017; M: 1Â Kb plus molecular weight maker. Sanger sequencing confirmed amplicon identities. Soybean cyclophilin (CYP2) reference gene was used as internal controls