Fig. 4
From: Detection and discovery of plant viruses in soybean by metagenomic sequencing
Symptoms caused by ClYVV-IA-2016 and ClYVV-IA-2017 in broad bean. Healthy mock-inoculated controls are indicated. N. benthamiana infected with ClYVV-IA-2016 or ClYVV-IA-2017 was used as an inoculum to infect broad bean plants by mechanical inoculation. A Whole plants (cv. Broad Windsor) infected with ClYVV IA-2016 and ClYVV IA-2017 at 21Â days post inoculation (dpi). B Systemic leaves (cv. Broad Windsor) showing symptoms at 21 dpi. C Whole plants (cv. Robin Hood) infected with ClYVV IA-2016 and ClYVV IA-2017 at 21 dpi. D Systemic leaves (cv. Robin Hood) showing symptoms at 21 dpi. (E) RT-PCR detection of ClYVV isolates in broad bean infected plants. Agarose gel electrophoresis of PCR confirming the presence of ClYVV-IA-2016 and ClYVV-IA-2017 identified by RNA sequencing. Lanes 1 and 4: Mock- inoculated controls of Broad Windsor and Robin Hood respectively; 2 and 5: Detection of ClYVV-IA-2016 in Broad Windsor and Robin Hood respectively; 3 and 6: Detection of ClYVV-IA-2017 in Broad Windsor and Robin Hood respectively; M: 1Â Kb plus molecular weight maker. Sanger sequencing confirmed amplicon identities. Broad bean cyclophilin (CYP2) reference gene was used as internal controls