Fig. 3
From: Detection and discovery of plant viruses in soybean by metagenomic sequencing
Symptoms of ClYVV-IA-2016 and ClYVV-IA-2017 in systemically infected leaves of N. benthamiana. Healthy mock-inoculated controls are indicated. Soybean field samples infected with ClYVV-IA-2016 or ClYVV-IA-2017 was used as an inoculum to infect N. benthamiana plants by mechanical inoculation. A Plants infected with ClYVV IA-2016 and ClYVV IA-2017 at 21Â days post inoculation (dpi). B Systemic leaf with mosaic chlorosis and epinasty symptoms at 21 dpi. C RT-PCR detection of ClYVV isolates in N. benthamiana infected plants. Agarose gel electrophoresis of PCR confirming the presence of ClYVV-IA-2016 and ClYVV-IA-2017 identified by RNA sequencing. Lane 1: Mock- inoculated control; 2: Detection of ClYVV-IA-2016; 3: Detection of ClYVV-IA-2017; M: 1Â Kb plus molecular weight maker. Sanger sequencing confirmed amplicon identities. N. benthamiana Protein phosphatase 2A (PP2A) reference gene was used as internal controls