Skip to main content
Fig. 5 | Virology Journal

Fig. 5

From: The role of N-linked glycosylation in proteolytic processing and cell surface transport of the Cedar virus fusion protein

Fig. 5

Analysis of N-linked carbohydrates in CedV F protein expressed at the cell surface of MDCK-2 cells. Surface biotinylated CedV F proteins were immunoprecipitated using NeutrAvidin beads and either left untreated (lanes “−”) or digested with endo-β-N-acetylglucosaminidase H (Endo H; lanes “+”). Samples were separated by SDS-PAGE under reducing conditions, and blots were analyzed using an antibody against the HA-tag (H6908), HRP-labeled secondary antibodies and chemiluminescence. Molecular masses of marker proteins are indicated. n = 2

Back to article page

Virology Journal

ISSN: 1743-422X