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Fig. 3 | Virology Journal

Fig. 3

From: The role of N-linked glycosylation in proteolytic processing and cell surface transport of the Cedar virus fusion protein

Fig. 3

Cell surface expression of CedV F proteins. At 24 h p.t., MDCK-2 cells expressing F proteins were surface-labeled with biotin on ice. After cell lysis, biotinylated proteins were immunoprecipitated using NeutrAvidin beads and subjected to SDS-PAGE under a non-reducing and b reducing conditions (n = 2). Precipitated F proteins were visualized using an antibody against the HA-tag (H6908), HRP-labeled secondary antibodies and chemiluminescence. In a, ConA staining is used as a loading control. Molecular masses of marker proteins are indicated

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Virology Journal

ISSN: 1743-422X