Table 1 Primers and probes sequences
From: Involvement of the Wnt pathway in BVDV cytopathogenic strain replication in primary bovine cells
Target (Accession number) | Foward(5'- > 3') | Reverse (5'- > 3') | Probe (5'- > 3') |
|---|---|---|---|
BVDV NADL (E2 region) | CCAAAGATGCACGAGAGAAACC | CTCTTGCTTTCGCCCATCAA | TGCATACAAGAGCCTTGCCGACCAGTGT |
TBP (NM0010745742) | GCGTTTTGCTGCTGTAATCA | GGAACTTCACATCACAGCTC | CGCACCACTGCACTGATATTCAGTTCT |
TLR2 (KT601038) | GTGCTTTGTGGACAGCATGG | TTGGACAGGTCAAGGCTTTTCA | CAGGCTTCTTCTCTGTCTTGTGACCC |
TLR3 (AJ812026) | AAAGGGACTTTGAGGCAGGT | GATTTAAACATYCCTCTTCG | ATGCAGTTCAGCAAGCTATTGAACAA |
NFkB (NM_001076409) | CTGGAAGCACGAATGACAGA | AACATGAGCCGTACCACGCT | CTATAATCCCGGACTTTT GGT GC A |
IL6 (EU276071) | CAAAATCTCTGCAATGAGAAAGG | GCTTCAGGATCTGGATCAGTG | GATGCTTCCAATCTGGGTTCAATC |
IL8 (AF232704) | ATGACTTCCAAGCTGGCTGTT | ACAACCTTCTGCACCCACTTT | CATTCCACACCTTTCCACCCCAAATTTATC |
Mxl (AF047692) | TTCCACCTGAAGAAGGGCTA | GCCATACTTCTGTAACTCCTCTGTT | ATCCCCTGCCTGGCAGAAAGACT |
ISG15 (Ml 74,366) | TGCAGAACTGC ATCTC CATC | CATGAACACGGTGCACCCCT | AGACCAGTTCTGGCTGTCTTTTGAAG |