Fig. 3

Preparation and characterization of the Nluc-ch2C5 antibody. A Analysis of recombinant plasmids by restriction endonuclease digestion. Lane 1, plasmid pcDNA3.1-ch2C5L digested with AflII and EcoRI. The inserted gene fragment, comprising the signal peptide and the ch2C5 light chain, is about 810 bp. Lane 2, plasmid pcDNA3.1-ch2C5H-Nluc digested by the same restriction endonucleases. The inserted gene fragment is about 2034 bp (signal peptide, ch2C5 heavy chain, linker, and Nluc). B Detection of luciferase activity in culture supernatant over 4 consecutive days. The light and heavy chain plasmids were co-transfected (at a 1:1 ratio) into COS-7 cells. Nanoluc-pcDNA3.1 (containing the Nluc gene only) and pcDNA3.1were used as negative controls. C Polyacrylamide gel electrophoresis of the purified Nluc-ch2C5 antibody. left: Coomassie blue staining; right: luciferase luminescence imaging analysis. D Binding of Nluc-ch2C5 to S-RBD.