Fig. 1

Maps of the vectors used for Nluc-ch2C5 production. A The pcDNA3.1-ch2C5L vector. The 810 bp fragment comprising a signal peptide sequence and the ch2C5 light chain was created by overlapping PCR and then inserted into pcDNA3.1 between the AflII and EcoRI sites to produce pcDNA3.1-ch2C5L. B pcDNA3.1-ch2C5H-Nluc. The 2034 bp gene fragment comprising the signal peptide, the ch2C5 heavy chain, an 18-mer linker, and Nluc was inserted into pcDNA3.1.