Skip to main content
Fig. 1 | Virology Journal

Fig. 1

From: The recombinant Erns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

Fig. 1

Expression and identification of CSFV Erns and BVDV tE2 proteins. A & C The recombinant CSFV Erns (A) or BVDV tE2 (C) protein expressed in E. Coli was analyzed by SDS-PAGE, with Coomassie blue staining. M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-Erns (A) or pET-tE2 (C); Lane 3, the purified CSFV Erns (A) or BVDV tE2 (C) protein. B & D Recombinant CSFV Erns (B) or BVDV tE2 (D) protein was confirmed by western blotting using an anti-His monoclonal antibody (upper) or a specific anti-virus polyclonal antibody (lower). M, molecular marker; Lane 1, lysate of bacteria harboring the control plasmid pET-28a; Lane 2, the lysate of bacteria harboring the plasmid pET-Erns (B) or pET-tE2 (D)

Back to article page

Virology Journal

ISSN: 1743-422X