Fig. 5
From: Swine influenza virus triggers ferroptosis in A549 cells to enhance virus replication
Effect of ferroptosis inhibitor on SIV replication and SIV-induced cytokine expression. A549 cells were treated with Fer-1 (1 μM) or not followed by infection with SIV at an MOI of 1. A Viral growth in A549 cells is reported as the TCID50 value 12, 24, 48 and 72 h.p.i.. B Cell lysates were collected at 12, 24 and 72 h.p.i. for western blot analysis of SIV NS1 protein levels. C IL-1β, TNF-α, IL-6, and IL-8 mRNA levels in the cell lysates were quantified by qRT–PCR. The error bars represent the SD of the means on the basis of three independent experiments (*p < 0.05, **p < 0.01)