Table 1 Primer and probe sequences used for RT-RAA and RT-qPCR assays
From: Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus
Primer | Sequence (5′–3′) | Size (bp) | Gene | Source |
|---|---|---|---|---|
H5-F | CAGTTTGAGGCYGTTGGAAGGGAATTTAAYAA | 32 | HA | This study |
H5-R | CTTGTCRTAAAGGTTCTTGACATTTGAGTCAT | 32 | HA | This study |
H5-P | CTAGATGTCTGGACTTATAATGCTGAACT/i6FAMdT//THF//iBHQ1dT/GGTTCTCATGGAAAAT[C3-spacer] | 47 | HA | This study |
H5 + 1456 | ACGTATGACTATCCACAATACTCAG | 25 | HA | [13] |
H5-1685 | AGACCAGCTACCATGATTGC | 20 | HA | [13] |
H5 + 1637 | FAM-TCAACAGTGGCGAGTTCCCTAGCA-TAMRA | 24 | HA | [13] |