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Table 2 Limit of detection and quantification

From: Development of a multiplex droplet digital PCR assay for detection of enterovirus, parechovirus, herpes simplex virus 1 and 2 simultaneously for diagnosis of viral CNS infections

  Virus
EV HPeV HSV1 HSV2
Meana 13.43 (10b) 13.25 (10) 37.80 (50) 12.68 (10)
SD 2.70 3.23 7.76 3.07
CV% 20.14 24.38 20.52 24.17
LOQc 10 10 50 10
LODd 5 10 5 10
  1. SD, standard deviation; CV, coefficient of variation
  2. aAverage of twenty replicates, in copies per reaction
  3. bExpected concentration determined by simplex ddPCR
  4. cLimit of quantification
  5. dLimit of detection
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Virology Journal

ISSN: 1743-422X