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Table 2 Limit of detection and quantification

From: Development of a multiplex droplet digital PCR assay for detection of enterovirus, parechovirus, herpes simplex virus 1 and 2 simultaneously for diagnosis of viral CNS infections

 

Virus

EV

HPeV

HSV1

HSV2

Meana

13.43 (10b)

13.25 (10)

37.80 (50)

12.68 (10)

SD

2.70

3.23

7.76

3.07

CV%

20.14

24.38

20.52

24.17

LOQc

10

10

50

10

LODd

5

10

5

10

  1. SD, standard deviation; CV, coefficient of variation
  2. aAverage of twenty replicates, in copies per reaction
  3. bExpected concentration determined by simplex ddPCR
  4. cLimit of quantification
  5. dLimit of detection
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Virology Journal

ISSN: 1743-422X