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Table 1 Primers used for detection and full-length genome amplification of Porcine circovirus type 3 (PCV3) in Tibetan pigs

From: Molecular detection and phylogenetic analysis of porcine circovirus type 3 in Tibetan pigs on the Qinghai-Tibet Plateau of China

Primers Sequences Binding positiona Lengthb Functionc
PCV3-D-1379-F 5′-CCATTCGTTTAGGCGGGTAATG-3′ 1379–1793 415 PCV3 detection
PCV3-D-1793-R 5′-CATTACCCGCCTAAACGAATGG-3′   
PCV3-CAP-1236-F 5′-ACATGCGAGGGCGTTTACCTGT-3′ 1236–1995 760 Genome sequencing of capsid
PCV3-CAP-1995-R 5′-GCACCAARATGAGACACAGAGCT-3′   
PCV3-genome-1-F 5′-TGGTGCTACGAGTGTCCTGAAGAT-3′ 171–1048 878 Amplify full-length PCV3 genomes
PCV3-genome-1-R 5′-CCTTRGTGAACCTCCTAAACAAGG-3′   
PCV3-genome-2-F 5′-ACCCTCTGAGGGTTCCTGTTAAG-3′ 902–1785 884
PCV3-genome-2-R 5′-GCCTAAACGAATGGGAAACTGC-3′   
PCV3-genome-3-F 5′-CAGGGCTGAGTGTAACTTTCATC-3′ 1711–230 520
PCV3-genome-3-R 5′-CACCGGACCTAGAATAGGATGA-3′