Table 1 Primers used for detection and full-length genome amplification of Porcine circovirus type 3 (PCV3) in Tibetan pigs
Primers | Sequences | Binding positiona | Lengthb | Functionc |
|---|---|---|---|---|
PCV3-D-1379-F | 5′-CCATTCGTTTAGGCGGGTAATG-3′ | 1379–1793 | 415 | PCV3 detection |
PCV3-D-1793-R | 5′-CATTACCCGCCTAAACGAATGG-3′ |  |  | |
PCV3-CAP-1236-F | 5′-ACATGCGAGGGCGTTTACCTGT-3′ | 1236–1995 | 760 | Genome sequencing of capsid |
PCV3-CAP-1995-R | 5′-GCACCAARATGAGACACAGAGCT-3′ |  |  | |
PCV3-genome-1-F | 5′-TGGTGCTACGAGTGTCCTGAAGAT-3′ | 171–1048 | 878 | Amplify full-length PCV3 genomes |
PCV3-genome-1-R | 5′-CCTTRGTGAACCTCCTAAACAAGG-3′ |  |  | |
PCV3-genome-2-F | 5′-ACCCTCTGAGGGTTCCTGTTAAG-3′ | 902–1785 | 884 | |
PCV3-genome-2-R | 5′-GCCTAAACGAATGGGAAACTGC-3′ |  |  | |
PCV3-genome-3-F | 5′-CAGGGCTGAGTGTAACTTTCATC-3′ | 1711–230 | 520 | |
PCV3-genome-3-R | 5′-CACCGGACCTAGAATAGGATGA-3′ |  |  |