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Fig. 1 | Virology Journal

Fig. 1

From: Multiplex detection of eight different viral enteropathogens in clinical samples, combining RT-PCR technology with melting curve analysis

Fig. 1

Amplification and melting curve of RRCMC assay. A Rotavirus C, astrovirus, rotavirus B, and norovirus GI were detected by FAM channel, and each virus was confirmed by temperature position of melting curve (Blue melting curve is the negative control and black melting curve is the sample). B Rotavirus A, sapovirus, norovirus GII, and adenovirus were detected by VIC channel, and each virus was confirmed by temperature position of melting curve (Green melting curve is the negative control and black melting curve is the sample). Amplication curve: ordinates is fluorescence value and abscissae is number of cycles; Melting curve: ordinates is Fluorescence value and abscissae is temperature

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