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Table 2 List of forward and reverse primers for PCR–RFLP of rs4343 and rs2285666 and their associated restriction enzymes and DNA fragments

From: SNPs of ACE1 (rs4343) and ACE2 (rs2285666) genes are linked to SARS-CoV-2 infection but not with the severity of disease

Polymorphism

Primer sequence (5′-3′)

TA (°C)

Restriction enzyme

DNA fragment size (bp)

References

A2350G (rs4343)

F-CTGACGAATGTGATGGCCGC

R-TTGATGAGTTCCACGTATTTCG

61

BstUI 60 °C/24 h

122/100/22

[52]

G8790A (rs2285666)

F-TTCTCCCTGCTCCTATACTACCG

R-TTCATTCATGTCCTTGCCCTTA

60

Alu1 37 °C/16 h

817/589/228

[53]

  1. TA, the temperature of annealing; bp, Base pair
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Virology Journal

ISSN: 1743-422X