Fig. 5

Effects of SVBV-encoded proteins on foreign gene expression driven by the SVBV SP1 promoter. A Analysis of GUS activity in N. benthamiana leaves..Agrobacterium cells harboring the pINT-SP1 vector were co-inoculated into N. benthamiana leaves with Agrobacterium cells harboring the pBIN-ORFI, pBIN-ORFII, pBIN-ORFIII, pBIN-ORFIV, pBIN-ORFV, pBIN-ORFVI, or pBIN438 (control) vector. The inoculated leaves were harvested at 64 hpi and then analyzed for GUS activity using fluorometric assays. Standard errors were determined using the LSD method. The biochemical expression assay in leaves was repeated thrice and average readings are represented. Statistical analysis showed a P value of < 0.05, indicating high significance. B Relative expression levels of the GUS gene in different tobacco plants. Total RNA was extracted from leaves harvested from tobacco plants separately co-inoculated with the pINT121 and pBIN-ORFV, pBIN-ORFVI, or pBIN438 (negative control) vector. Relative GUS mRNA levels were determined by RT-qPCR using GUS gene-specific primers. The relative expression level of the tobacco β-actin gene was used as an internal control. Standard errors were determined using the LSD method. The biochemical expression assay in leaves was repeated thrice and average readings are represented. Statistical analysis showed a P value of < 0.05, indicating high significance