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Fig. 6 | Virology Journal

Fig. 6

From: The long non-coding RNA LNC_000397 negatively regulates PRRSV replication through induction of interferon-stimulated genes

Fig. 6

a PAMs were either mock-infected or infected with GSWW or VR2332 (MOI = 0.1). At 12, 24 and 36 hpi, the LNC_000397 levels were determined by qRT-PCR. b PAMs were either mock-infected or infected with the VR2332 at MOIs of 0.05, 0.1, 0.5. At 24 hpi, the LNC_000397 mRNA levels were determined by qRT-PCR. c PAMs were mock-infected or infected with GSWW or VR2332 (MOI = 0. 1), UV-inactivated, heat-inactivated GSWW, or VR2332. LNC_000397 were quantified by qRT-PCR at 24 hpi. d and e PAMs and PK-15 cells were transfected with 2 μg/ml poly(I: C), 24 h after transfection, the LNC_000397 mRNA levels were determined by qRT-PCR. The bar represents the mean of three independent experiments. Expression levels were normalized to GAPDH

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