Table 3 Transition/transversion and non-synonymous mutations of viruses passaged for 5 generations
Virus strain | Origination of the 5th generation | SBS Count | Ts | Tv | PTs | PTv | Ratio of PTs and PTv | Non-synonymous count | Non-synonymous percentagea | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Run1 | Run2 | Run1 | Run2 | Run1 | Run2 | Run1 | Run2 | Run1 | Run2 | Run1 | Run2 | Run1 | Run2 | Run1 | Run2 | ||
A2093 | MDCK | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | na | na | 0 | 0 | na | na |
WSN | MDCK | 3 | 9 | 3 | 9 | 0 | 0 | 0.0686 | 0.199 | 0 | 0 | na | na | 1 | 5 | 33.33% | 55.56% |
A2093HANAPB1/WSN | MDCK | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0.181 | 0.225 | 0 | 0 | na | 1 | 0 | 100.00% | 0.00% |
A2093HANAPA/WSN | MDCK | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | na | na | 0 | 0 | na | na, |
A2093HANANP/WSN | MDCK | 2 | na | 1 | na | 1 | na | 0.0174 | na | 0.492 | na | 0.035 | na | 1 | na | 50.00% | na |
A2093 | MOUSE LUNG | 10 | 6 | 5 | 4 | 5 | 2 | 0.2446 | 0.228 | 0.109 | 0.074 | 2.249 | 3.092 | 10 | 6 | 100.00% | 100.00% |
WSN | MOUSE LUNG | 18 | 13 | 11 | 13 | 7 | 0 | 0.33 | 0.362 | 0.069 | 0 | 4.769 | na | 14 | 9 | 77.78% | 69.23% |
A2093HANAPB1/WSN | MOUSE LUNG | 14 | 10 | 7 | 7 | 7 | 3 | 0.2554 | 0.249 | 0.811 | 0.771 | 0.315 | 0.323 | 11 | 8 | 78.57% | 80.00% |
A2093HANAPA/WSN | MOUSE LUNG | 4 | 3 | 3 | 3 | 1 | 0 | 0.9448 | 0.949 | 0.014 | 0 | 67.14 | na | 4 | 3 | 100.00% | 100.00% |
A2093HANANP/WSN | MOUSE LUNG | 7 | 5 | 2 | 2 | 5 | 3 | 0.0986 | 0.093 | 0.092 | 0.068 | 1.07 | 1.375 | 6 | 4 | 85.71% | 80.00% |