Fig. 4

Silencing of Nrf2 promotes virus replication. HEp-2 cells were pretreated with Nrf2 siRNA (100 nM) or NC siRNA (100 nM) 12 h before HSV-1 (MOI = 1) infection, HEp-2 cells infected HSV-1 were used as positive control and untreated HEp-2 cells were used as negative control. Cells were cultured in maintenance medium for 24 h, then protein and total RNA were extracted. a The expression of Nrf2, HO-1, NQO1 and virus protein gD were identified by Western Blot. b Cells were repeatedly frozen and thawed for 3 times, then the viral titer of the supernatant was measured by TCID₅₀ assay. Data represent the results from three independent experiments (Mean ± SD, **P < 0.01, ns, not significant)