Fig. 3

IRF2 silencing decreased FAM111A expression and knockdown of FAM111A or RFC3 promoted ZIKV replication in both 2FTGH and U5A cells. 2FTGH and U5A cells were transfected with siNC or siIRF2 for 24 h, and then the cells were infected with ZIKV at MOI of 0.5. Expression levels of FAM111A were examined both at mRNA (a, b) and protein (c, d) levels 48 h post transfection. 2FTGH and U5A cells were transfected with siNC, siFAM111A, or siRFC3 for 24 h, and then the cells were infected with ZIKV at MOI of 0.5. Expression levels of ZIKV-NS5 were examined both at RNA (e, f) and protein levels (g, h) 48 h post transfection. ISG15 and IFIT1 were examined at mRNA levels with RT-qPCR (i–l). The relative ZIKV production in 2FTGH cells between siNC and siFAM111A (P = 0.03, F = 15.2) or siRFC3 (P = 0.004, F = 68.9) were significantly different. The relative ZIKV production in U5A cells between siNC and siFAM111A (P = 0.002, F = 90.1) or siRFC3 (P = 0.001, F = 145.6) were significantly different. Data were presented as mean ± standard deviation representative of at least three independent experiments (t test, *P < 0.05; **P < 0.01)