Fig. 4
ZIKV and WNVKUN viral protein localisation in HTR8/SVneo and BeWo cells. Viral envelope protein (E) expression and distribution was assessed by IFA at 48 h.p.i. in BeWo (A) or HTR8/SVneo (B) cells. Mock-infected (panels Ai-iii and Bi-iii), WNVKUN (panels Aiv-vi and Biv-vi) and ZIKV (panels Avii-ix and Bvii-ix) E expression was visualised with anti-envelope antibodies and species-specific IgG conjugated to AF488 (green; panels Ai, iv, vii and Bi, iv, vii), and the nuclei were counterstained with dapi (blue; panels Aii, v, vii and Bii, v, vii). Merged images are in the right-hand panels (Aiii, vi, ix and Biii, vi, ix) as indicated. Images were processed and assembled in Adobe Photoshop