Fig. 1

T cell responses to AdC-gDPolN, AdC-gDPolC and AdC-gDCore. Male C57Bl/6 mice (n = 5) were injected with the indicated doses of the AdC6-gDPolC, AdC7-gDPolC, AdC6-gDPolN, AdC7-gDPolN, AdC6-gDCore or AdC7-gDCore vectors. Naïve mice served as controls. a, b Fourteen days later PMBCs were tested by ICS for CD8⁺ and CD4⁺ T cells producing IFN-γ in response to peptide pools reflecting the HBV inserts. Data are shown for individual mice with lines indicating medians. Lines with stars above indicate significant differences by 2-way Anova. (*) p ≤ 0.05–0.01, (**) p ≤ 0.01–0.001, (***) p ≤ 0.001–0.0001, (****) p ≤ 0.0001. c Mice were immunized with the indicated doses of AdC6-gDPolN. PBMCs were tested at different times after vaccination by ICS. Naïve mice served as controls. Graph shows medians ± IQR. Lines with stars above indicate significant differences within one vaccine group tested at different times by one-way ANOVA. d Groups of C57Bl/6 mice were primed with the indicated doses of AdC6-gDPolN. PBMCs were tested 8 weeks later. The next day mice were boosted with the same doses of the AdC7-gDPolN vector. PBMCs were tested 2 weeks later. Graph shows frequencies of IFN-γ producing CD8⁺ T cells from individual mice. Lines with stars above indicate significant differences by two-way ANOVA