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Table 2 AIV spreading activity of freshwater crabs

From: Freshwater crabs could act as vehicles of spreading avian influenza virus

Hours post co-cultured (hpc)

Viral titer (log10TCID50/mL)a

Inoculated crabs

Sentinel crabs

Water

0

4.96/4.91/4.61

–

–

0.5

4.05/3.71/3.67

–

–

1

3.74/3.62/3.18

2.89/2.34/–

2.50

1.5

3.84/2.85/2.35

2.95/2.45/–

2.25

2

3.68/3.17/2.13

2.60/2.35/–

–

2.5

3.24/2.68/2.16

2.68/–/–

–

3

2.49/2.32/–

–

–

3.5

2.70/–/–

–

–

4

2.57/–/–

–

–

8

2.49/–/–

–

–

12 to 60*

–

–

–

  1. aGroups of 3 crabs were inoculated by incubating in viral water for 8 h. After rinsing thoroughly, the inoculated crabs were transferred into fresh water tanks. Groups of 3 sentinel crabs were put into each tank. At designated time points, 1 group of inoculated and sentinel crabs were rinsed and euthanized. Their gills and water were collected at the same time for viral titration in MDCK cells.
  2. –, no virus was detected in samples
  3. *Crabs and water samples were collected every 12 h
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Virology Journal

ISSN: 1743-422X