Fig. 2

Comparative analysis of serum markers and HBV quasispecies characteristics between 8 pairs of mothers and infants at 7 months and 3 years old. Dynamic change of serum markers and HBV quasispecies characteristics at full-length HBV genome level (A). The quasispecies complexities (B), mutation frequencies (C) and genetic distances (D) of Core, PreS2, P, RT, S and NTCP-BD regions at nucleotide level at three time points. The quasispecies complexities (E), mutation frequencies (F) and genetic distances (G) of Core, PreS2, P and RT regions at amino acid level at three time points. The dynamic change of synonymous substitution rates (dS) (H) and the non-synonymous substitution rates (dN) (I) of Core, PreS2, P, RT, S and NTCP-BD regions at three time points. The phylogenetic trees of 8 mothers and their paired infants at 7 months and 3 years old. (J) Black point represents the clones from mothers, light blue point for 7-month-old infants and dark blue point for 3-year-old infants. *represents P < 0.05. “nt” represents nucleotide level. “aa” represents amino acid level. NTCP-BD: sodium taurocholate cotransporting polypetide binding domain; Quasispecies complexity was measured using normalized Shannon entropy (Sn). Genetic distance was calculated at the nucleotide level under Tamura 3-parameter method and at the amino acid level under Jones–Taylor–Thornton matrix-based method. dS and dN were calculated under modified Nei–Gojobori model with Jukes–Cantor correction