Table 2 Initial diagnosis PCR protocols and primers used
Target gene | Primer sequences (5′-3′) | Product length (bp) | References |
|---|---|---|---|
Terminase | Round 1: TS-TERM_707s: TTGTGGACGAGRSIMAYTTYAT TS-TERM_707as: ACAGCCACGCCNGTICCIGAIGC Round 2: TS-TERM_708s: GCAAGATCATNTTYRTITCITC TS-TERM_708as: TGTTGGTCGTRWAIGCIGGRT | Round 1: 519 Round 2: 419 | [29] |
Polymerase | Round 1: DFA: GAYTTYGCNAGYYTNTAYCC KG1: GTCTTGCTCACCAGNTCNACNCCYTT ILK: TCCTGGACAAGCAGCARNYSGCNMTNAA KG1: GTCTTGCTCACCAGNTCNACNCCYTT Round 2: TGV: TGTAACTCGGTGTAYGGNTTYACNGGNGT IYG: CACAGAGTCCGTRTCNCCRTADAT | Round 1: DFA/KGI: ~ 750 ILK/KGI: ~ 480 Round 2: 215–315 | [30] |
EEHV DNA Polymerase- Specific EEHV 3/4 | 6719: CGTTGAAGGTGTCGCAGAT 7400: CAGCATCATCCAGGCCTACAAC 6720: ATCCTGGCGCAGCTGCTGAC 6721: CTCACCTGCAACGCCGTCTA | Round 1: 6719/7400: 390 Round 2: 6719/6720: 270 Round 3: 6719/6721: 150 | [13] |
EEHV DNA Polymerase- PAN EEHV and specific EEHV 6 | 6710: ACAAACACGCTGTCRGTRTCYCCRTA 6711: GTATTTGATTTYGCNAGYYTGTAYCC 6712: TGYAAYGCCGTNTAYGGATTYACCGG 7584: CATCGATTTTGAACTTCTCATGGTC | Round 1: 6710/6711: 500 Round 2A: 6710/6712: 250 Round 2B: 6711/7584: 500 Round 3A: 6712/7584: 250 | [13] |
EEHV DNA Terminase | B1 LGH2425: ACAGCCACGCCNGTNCCNGANGC A2 LGH2428: TTGTGGACGAGRSNMAYTTYAT A3 LGH2426: GCAAGATCATNTTYRTNTCNTC B2 LGH2427: TGTTGGTCGTRWANGCNGGRTC A3SEQ: CCCCATCTGAGCAAGATCAT B2SEQ: GGCTGACAAATGTTGGTCGT | Round 1: B1/A2: 575 Round 2: A3/B2: 415 A3SEQ/B2SEQ: 360 | [4] |