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Fig. 10 | Virology Journal

Fig. 10

From: High throughput sequencing of whole transcriptome and construct of ceRNA regulatory network in RD cells infected with enterovirus D68

Fig. 10

Effects of overexpression of Fos and ARRDC3 on EV-D68 replication. CD513B-Fos (CD513B-NC) or CD513B-ARRDC3 (CD513B-NC) were transfected into RD cells, which were then infected with EV-D68 at a multiplicity of infection (MOI) of 1 after 48 h. RT-qPCR was used to evaluate the quantities of EV-D68 viral VP1 RNA in the Fos overexpression group (a) and the ARRDC3 overexpression group (b). At the indicated time points after infection, virus titers in culture supernatants from the Fos overexpression group (c) and the ARRDC3 overexpression group (d) were measured. The protein expression of VP1 in Fos overexpression group (e) and ARRDC3 overexpression group (f) were determined by western blot assay. *p < 0.05, **p < 0.01, ***p < 0.001

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Virology Journal

ISSN: 1743-422X