Fig. 4

lymphoproliferative activity of splenocytes after in vitro stimulation with γ-PR8 (4 μg/ml) as the specific antigen. One week following the third immunization, the supernatants from restimulated spleen cells were collected and then subjected to lymphocyte proliferation analysis using the MTT assay. Values are presented as mean ± SD of three tested mice in each group. The levels of significance are determined by One way ANOVA and indicated by stars as follows: * p ˂ 0.05, *** p ˂ 0.001. Cell proliferation of the γ-Flu-CCL21-Poly (I:C) group was significantly higher than those in other vaccinated and control groups (p < 0.001)