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Fig. 3 | Virology Journal

Fig. 3

From: Antigen-capture ELISA and immunochromatographic test strip to detect the H9N2 subtype avian influenza virus rapidly based on monoclonal antibodies

Fig. 3

Assessment of AC-ELISA. a To determine the specificity, samples of 100 μl of the H9N2 subtype AIVs or non-H9N2 viruses (OD value) allantoic fluid were measured and averaged from three independent tests. b To test the sensitivity, three different H9N2 subtype AIVs and one H10N8 subtype AIV (negative control) at an HA titer of 24 were twofold serially diluted. c Standard curve of AC-ELISA for the purified H9 HA protein (A/chicken/Zhejiang/329/2011). The limit of detection was 31.5 ng/ml. The OD was measured at 450 nm, and the cut-off value is denoted by a dotted line

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Virology Journal

ISSN: 1743-422X