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Fig. 2 | Virology Journal

Fig. 2

From: GATA binding protein 4 promotes the expression and transcription of hepatitis B virus by facilitating hepatocyte nuclear factor 4 alpha in vitro

Fig. 2

Overexpression of GATA4 may promote HBV in L02 cells and SMMC7721 cells, respectively. L02 cells and SMMC7721 cells were transfected HBV cccDNA (500 ng per 1 mL 1640 medium, as 500 ng/mL) and adGATA4 for 48 h. (Description for the final concentration of adGATA4: − , control; + , 100 ng/mL; ++ , 200 ng/mL; +++ , 400 ng/mL.) a Relative expression of GATA4 mRNA in cells detected via RT-qPCR, β-actin was used as the internal control. b Analysis of HBV DNA copies in cells determined by qPCR. c HBsAg and HBeAg levels in the culture medium detected through enzyme-linked immunosorbent assays. d Relative levels of HBV 3.5 kb pgRNA and total RNA in cells analyzed via RT-qPCR, β-actin was used as the internal control. e Protein of GATA4 (51 kDa) analyzed via WB. All statistics are marked as NS P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001

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Virology Journal

ISSN: 1743-422X