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Table 1 List of chikungunya primers and PCR cycling parameters

From: Detection of chikungunya virus in the Southern region, Saudi Arabia

Primers

Sequence

Size (bp)

Forward CHIK 1

AAGCTCCGCGTCCTTTACCAAG

208

Reverse CHIK 1

CCAAATTGTCCTGGTCTTCCT

Cycling parameters

Step

Cycles

Temperature

Time

Initial Denaturation

1

95

1 min

Denaturation

35

95

15 s

Annealing

55

15 s

Extension

72

10 s

Final extension

1

72

10 s

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Virology Journal

ISSN: 1743-422X